Composite

Part:BBa_K4432121:Design

Designed by: Paul Weimer   Group: iGEM22_Evry_Paris-Saclay   (2022-09-26)


CrtO expression cassette under the control of the T5 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 569
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T5 promoter (BBa_K4432000) + custom made RBS (BBa_K4432011) + CrtO coding sequence (BBa_K4432021)

Source

DNA synthesis, PCR amplification & Assembly

References